ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (9): 1526-1530.doi: 10.11843/j.issn.0366-6964.2014.09.020

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The Damage Mechanism of Neuroblastoma Cells Mitochondria in the Presence of PrP106-126 Peptide

YUAN Fang-zhong1,GENG Mei-ying1,ZHAO De-ming2*   

  1. (1.College of Veterinary Medicine,Hebei Agricultural University,Baoding 071001,China;2.National Animal Transmissible Spongiform Encephalopathy Laboratory,College of Veterinary  Medicine,China Agricultural University,Beijing 100193,China)
  • Received:2014-02-13 Online:2014-09-23 Published:2014-09-23

Abstract:

 The purpose of this study was to investigate the possible mechanism of neuron cell damage in Prion diseases.And we studied the respiratory chain function of N2a cells by testing the NADH activity after treatment with ferric ions and PrP106-126,as well as the depolarization of the mitochondrial membrane potential and the distribution of cytochrome C.It is proved that the activity of NADH of N2a cells,which were treated with ferric ions and PrP106-126,was significantly decreased (P <0.01) to about 65%.And the mitochondrial membrane potential of N2a cells had been depolarization significantly.It is showed that the cytochrome C were released into cytoplasm and activated the pro-caspase-9.

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